Escherichia coli "TatExpress" strains export several g/L human growth hormone to the periplasm by the Tat pathway.

ABSTRACT: Escherichia coli is a heavily used platform for the production of biotherapeutic and other high-value proteins, and a favored strategy is to export the protein of interest to the periplasm to simplify downstream processing and facilitate disulfide bond formation. The Sec pathway is the standard means of transporting the target protein but it is unable to transport complex or rapidly folding proteins because the Sec system can only transport proteins in an unfolded state. The Tat system also operates to transport proteins to the periplasm, and it has significant potential as an alternative means of recombinant protein production because it transports fully folded proteins. Here, we have tested the Tat system's full potential for the production of biotherapeutics for the first time using fed

SUBMITTER: Guerrero Montero I 

PROVIDER: S-EPMC6907408 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature