In-depth comparison of cell-based methodological approaches to determine drug susceptibility of visceral Leishmania isolates.

ABSTRACT: Monitoring the drug susceptibility of Leishmania isolates still largely relies on standard in vitro cell-based susceptibility assays using (patient-isolated) promastigotes for infection. Although this assay is widely used, no fully standardized/harmonized protocol is yet available hence resulting in the application of a wide variety of host cells (primary cells and cell lines), different drug exposure times, detection methods and endpoint criteria. Advocacy for standardization to decrease inter-laboratory variation and improve interpretation of results has already repeatedly been made, unfortunately still with unsatisfactory progress. As a logical next step, it would be useful to reach at least some agreement on the type of host cell and basic experimental design for routine amastigote sus

SUBMITTER: Hendrickx S 

PROVIDER: S-EPMC6907865 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature