Calretinin positive neurons form an excitatory amplifier network in the spinal cord dorsal horn.

ABSTRACT: Nociceptive information is relayed through the spinal cord dorsal horn, a critical area in sensory processing. The neuronal circuits in this region that underpin sensory perception must be clarified to better understand how dysfunction can lead to pathological pain. This study used an optogenetic approach to selectively activate spinal interneurons that express the calcium-binding protein calretinin (CR). We show that these interneurons form an interconnected network that can initiate and sustain enhanced excitatory signaling, and directly relay signals to lamina I projection neurons. Photoactivation of CR interneurons in vivo resulted in a significant nocifensive behavior that was morphine sensitive, caused a conditioned place aversion, and was enhanced by spared nerve injury. Furthermore

SUBMITTER: Smith KM 

PROVIDER: S-EPMC6908433 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature