SureSelect targeted enrichment, a new cost effective method for the whole genome sequencing of Candidatus Liberibacter asiaticus.

ABSTRACT: Huanglongbing (HLB) is a worldwide deadly citrus disease caused by the phloem-limited bacteria 'Candidatus Liberibacter asiaticus' (CLas) vectored by Asian citrus psyllids. In order to effectively manage this disease, it is crucial to understand the relationship among the bacterial isolates from different geographical locations. Whole genome sequencing approaches will provide more precise molecular characterization of the diversity among populations. Due to the lack of in vitro culture, obtaining the whole genome sequence of CLas is still a challenge, especially for medium to low titer samples. Hundreds of millions of sequencing reads are needed to get good coverage of CLas from an HLB positive citrus sample. In order to overcome this limitation, we present here a new method, Agilent SureS

SUBMITTER: Cai W 

PROVIDER: S-EPMC6908597 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature