Downstream Processing of Chlamydomonas reinhardtii TN72 for Recombinant Protein Recovery.

ABSTRACT: The green microalga Chlamydomonas reinhardtii is under development as a production host for recombinant proteins and whole-cell therapeutics. In particular, the cell wall-reduced strain TN72 is used as a model organism for protein expression and algal synthetic biology. However, the bioprocessing characteristics of TN72 and other C. reinhardtii strains have yet to be examined. Here we use a TN72 strain expressing a protein-based antibiotic (Pal) to study the scale-up of cell harvest and product recovery. Cell harvest was examined with 100L cultures in two intermittent-discharge continuous-flow disc-stack centrifuges at flow rates of 150-250 L.h^-1, as well as with an ultra scale-down (USD) mimic of the centrifuges. Solids recovery exceeded 99.5% and the loss of prod

SUBMITTER: Stoffels L 

PROVIDER: S-EPMC6908742 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature