Sequential Co-immobilization of Enzymes in Metal-Organic Frameworks for Efficient Biocatalytic Conversion of Adsorbed CO₂ to Formate.

ABSTRACT: The main challenges in multienzymatic cascade reactions for CO₂ reduction are the low CO₂ solubility in water, the adjustment of substrate channeling, and the regeneration of co-factor. In this study, metal-organic frameworks (MOFs) were prepared as adsorbents for the storage of CO₂ and at the same time as solid supports for the sequential co-immobilization of multienzymes via a layer-by-layer self-assembly approach. Amine-functionalized MIL-101(Cr) was synthesized for the adsorption of CO₂. Using amine-MIL-101(Cr) as the core, two HKUST-1 layers were then fabricated for the immobilization of three enzymes chosen for the reduction of CO₂ to formate. Carbonic anhydrase was encapsulated in the inner HKUST-1 layer and hydrated the release

SUBMITTER: Li Y 

PROVIDER: S-EPMC6908815 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature