Genome-Resolved Proteomic Stable Isotope Probing of Soil Microbial Communities Using ¹³CO₂ and ¹³C-Methanol.

ABSTRACT: Stable isotope probing (SIP) enables tracking the nutrient flows from isotopically labeled substrates to specific microorganisms in microbial communities. In proteomic SIP, labeled proteins synthesized by the microbial consumers of labeled substrates are identified with a shotgun proteomics approach. Here, proteomic SIP was combined with targeted metagenomic binning to reconstruct metagenome-assembled genomes (MAGs) of the microorganisms producing labeled proteins. This approach was used to track carbon flows from ¹³CO₂ to the rhizosphere communities of Zea mays, Triticum aestivum, and Arabidopsis thaliana. Rhizosphere microorganisms that assimilated plant-derived ¹³C were capable of metabolic and signaling interactions with their plant

SUBMITTER: Li Z 

PROVIDER: S-EPMC6908837 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature