Divergent Biosynthesis of C-Nucleoside Minimycin and Indigoidine in Bacteria.

ABSTRACT: Minimycin (MIN) is a C-nucleoside antibiotic structurally related to pseudouridine, and indigoidine is a naturally occurring blue pigment produced by diverse bacteria. Although MIN and indigoidine have been known for decades, the logic underlying the divergent biosynthesis of these interesting molecules has been obscure. Here, we report the identification of a minimal 5-gene cluster (min) essential for MIN biosynthesis. We demonstrated that a non-ribosomal peptide synthetase (MinA) governs "the switch" for the divergent biosynthesis of MIN and the cryptic indigoidine. We also demonstrated that MinC_N (the N-terminal phosphatase domain of MinC), MinD (uracil phosphoribosyltransferase), and MinT (transporter) function together as the safeguard enzymes, which collaboratively constit

SUBMITTER: Kong L 

PROVIDER: S-EPMC6908994 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature