Process engineering of pH tolerant Ustilago cynodontis for efficient itaconic acid production.

ABSTRACT: BACKGROUND:Ustilago cynodontis ranks among the relatively unknown itaconate production organisms. In comparison to the well-known and established organisms like Aspergillus terreus and Ustilago maydis, genetic engineering and first optimizations for itaconate production were only recently developed for U. cynodontis, enabling metabolic and morphological engineering of this acid-tolerant organism for efficient itaconate production. These engineered strains were so far mostly characterized in small scale shaken cultures. RESULTS:In pH-controlled fed-batch experiments an optimum pH of 3.6 could be determined for itaconate production in the morphology-engineered U. cynodontis Δfuz7. With U. cynodontis ∆fuz7r ∆cyp3r PetefmttA Pria1ria1, optimized for itaconate production through the deletion of

SUBMITTER: Hosseinpour Tehrani H 

PROVIDER: S-EPMC6909570 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature