DNA methylation data by sequencing: experimental approaches and recommendations for tools and pipelines for data analysis.

ABSTRACT: Sequencing technologies have changed not only our approaches to classical genetics, but also the field of epigenetics. Specific methods allow scientists to identify novel genome-wide epigenetic patterns of DNA methylation down to single-nucleotide resolution. DNA methylation is the most researched epigenetic mark involved in various processes in the human cell, including gene regulation and development of diseases, such as cancer. Increasing numbers of DNA methylation sequencing datasets from human genome are produced using various platforms-from methylated DNA precipitation to the whole genome bisulfite sequencing. Many of those datasets are fully accessible for repeated analyses. Sequencing experiments have become routine in laboratories around the world, while analysis of outcoming data

SUBMITTER: Rauluseviciute I 

PROVIDER: S-EPMC6909609 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature