STAT3 Modulation of Regulatory T Cells in Response to Radiation Therapy in Head and Neck Cancer.

ABSTRACT:

Background

Radioresistance represents a major problem in the treatment of head and neck cancer (HNC) patients. To improve response, understanding tumor microenvironmental factors that contribute to radiation resistance is important. Regulatory T cells (Tregs) are enriched in numerous cancers and can dampen the response to radiation by creating an immune-inhibitory microenvironment. The purpose of this study was to investigate mechanisms of Treg modulation by radiation in HNC.

Methods

We utilized an orthotopic mouse model of HNC. Anti-CD25 was used for Treg depletion. Image-guided radiation was delivered to a dose of 10 Gy. Flow cytometry was used to analyze abundance and function of intratumoral immune cells. Enzyme-linked immunosorbent assay was performed to assess secreted

SUBMITTER: Oweida AJ 

PROVIDER: S-EPMC6910208 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature