Multiple roles of Bet v 1 ligands in allergen stabilization and modulation of endosomal protease activity.

ABSTRACT:

Background

Over 100 million people worldwide suffer from birch pollen allergy. Bet v 1 has been identified as the major birch pollen allergen. However, the molecular mechanisms of birch allergic sensitization, including the roles of Bet v 1 and other components of the birch pollen extract, remain incompletely understood. Here, we examined how known birch pollen-derived molecules influence the endolysosomal processing of Bet v 1, thereby shaping its allergenicity.

Methods

We analyzed the biochemical and immunological interaction of ligands with Bet v 1. We then investigated the proteolytic processing of Bet v 1 by endosomal extracts in the presence and absence of ligands, followed by a detailed kinetic analysis of Bet v 1 processing by individual endolysosomal proteases as we

SUBMITTER: Soh WT 

PROVIDER: S-EPMC6910946 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature