HIPP1 stabilizes the interaction between CP190 and Su(Hw) in the Drosophila insulator complex.

ABSTRACT: Suppressor of Hairy-wing [Su(Hw)] is one of the best characterized architectural proteins in Drosophila and recruits the CP190 and Mod(mdg4)-67.2 proteins to chromatin, where they form a well-known insulator complex. Recently, HP1 and insulator partner protein 1 (HIPP1), a homolog of the human co-repressor Chromodomain Y-Like (CDYL), was identified as a new partner for Su(Hw). Here, we performed a detailed analysis of the domains involved in the HIPP1 interactions with Su(Hw)-dependent complexes. HIPP1 was found to directly interact with the Su(Hw) C-terminal region (aa 720-892) and with CP190, but not with Mod(mdg4)-67.2. We have generated Hipp1 null mutants (Hipp^Δ1) and found that the loss of Hipp1 does not affect the enhancer-blocking or repression activities of the Su(Hw)-de

SUBMITTER: Melnikova L 

PROVIDER: S-EPMC6911044 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature