Neogenin-1 distinguishes between myeloid-biased and balanced Hoxb5⁺ mouse long-term hematopoietic stem cells.

ABSTRACT: Hematopoietic stem cells (HSCs) self-renew and generate all blood cells. Recent studies with single cell transplants and lineage tracing suggest that adult HSCs are diverse in their reconstitution and lineage potentials. However, prospective isolation of these subpopulations has remained challenging. Here, we identify Neogenin-1 (NEO1) as a unique surface marker on a fraction of mouse HSCs labeled with Hoxb5, a specific reporter of long-term HSCs (LT-HSCs). We show that NEO1⁺Hoxb5⁺ LT-HSCs expand with age and respond to myeloablative stress in young mice while NEO1^-Hoxb5⁺ LT-HSCs exhibit no significant change in number. Furthermore, NEO1⁺Hoxb5⁺ LT-HSCs are more often in the G₂/S cell cy

SUBMITTER: Gulati GS 

PROVIDER: S-EPMC6911217 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature