Interleukin-15 Enhances Anti-GD2 Antibody-Mediated Cytotoxicity in an Orthotopic PDX Model of Neuroblastoma.

ABSTRACT:

Purpose

Immunotherapy with IL2, GM-CSF, and an anti-disialoganglioside (GD2) antibody significantly increases event-free survival in children with high-risk neuroblastoma. However, therapy failure in one third of these patients and IL2-related toxicities pose a major challenge. We compared the immunoadjuvant effects of IL15 with those of IL2 for enhancing antibody-dependent cell-mediated cytotoxicity (ADCC) in neuroblastoma.

Experimental design

We tested ADCC against neuroblastoma patient-derived xenografts (PDX) in vitro and in vivo and examined the functional and migratory properties of NK cells activated with IL2 and IL15.

Results

In cell culture, IL15-activated NK cells induced higher ADCC against two GD⁺ neuroblastoma PDXs than did IL2-ac

SUBMITTER: Nguyen R 

PROVIDER: S-EPMC6911623 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature