High-Throughput Platform for Optoacoustic Probing of Genetically Encoded Calcium Ion Indicators.

ABSTRACT: Functional optoacoustic (OA) imaging assisted with genetically encoded calcium ion indicators (GECIs) holds promise for imaging large-scale neuronal activity at depths and spatiotemporal resolutions not attainable with existing optical microscopic techniques. However, currently available GECIs optimized for fluorescence (FL) imaging lack sufficient contrast for OA imaging and respond at wavelengths having limited penetration into the mammalian brain. Here we present an imaging platform capable of rapid assessment and cross-validation between OA and FL responses of sensor proteins expressed in Escherichia coli colonies. The screening system features optimized pulsed light excitation combined with ultrasensitive ultrasound detection to mitigate photobleaching while further allowing the dynam

SUBMITTER: Hofmann UAT 

PROVIDER: S-EPMC6911978 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature