Targeted RNA-sequencing assays: a step forward compared to FISH and IHC techniques?

ABSTRACT:

Introduction

ALK and ROS1 rearrangements are molecular targets of several tyrosine kinase inhibitors. RNA-sequencing approaches are regarded as the new standard for fusion gene detection, representing an alternative to standard immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) techniques.

Patients and methods

We aimed to compare two recent amplicon-based RNA-sequencing techniques: FusionPlex^® Alk Ret Ros1 v2 Kit (Archer^® ) with FHS-003Z-12-Human Lung Cancer Panel (Qiagen^® ) and assessed the accuracy of the data for therapy management. Thirty-seven formalin-fixed paraffin-embedded non-small cell carcinoma (NSCC) lesions initially explored by IHC and FISH were selected for RNA-sequencing analysis.

Results

Qiagen

SUBMITTER: Tachon G 

PROVIDER: S-EPMC6912030 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature