Clonal Decomposition and DNA Replication States Defined by Scaled Single-Cell Genome Sequencing.

ABSTRACT: Accurate measurement of clonal genotypes, mutational processes, and replication states from individual tumor-cell genomes will facilitate improved understanding of tumor evolution. We have developed DLP+, a scalable single-cell whole-genome sequencing platform implemented using commodity instruments, image-based object recognition, and open source computational methods. Using DLP+, we have generated a resource of 51,926 single-cell genomes and matched cell images from diverse cell types including cell lines, xenografts, and diagnostic samples with limited material. From this resource we have defined variation in mitotic mis-segregation rates across tissue types and genotypes. Analysis of matched genomic and image measurements revealed correlations between cellular morphology and genome plo

SUBMITTER: Laks E 

PROVIDER: S-EPMC6912164 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature