A Role for H₂O₂ and TRPM2 in the Induction of Cell Death: Studies in KGN Cells.

ABSTRACT: Recent studies showed that KGN cells, derived from a human granulosa cell tumor (GCT), express NADPH oxidase 4 (NOX4), an important source of H₂O₂. Transient receptor potential melastatin 2 (TRPM2) channel is a Ca²⁺ permeable cation channel that can be activated by H₂O₂ and plays an important role in cellular functions. It is also able to promote susceptibility to cell death. We studied expression and functionality of TRPM2 in KGN cells and examined GCT tissue microarrays (TMAs) to explore in vivo relevance. We employed live cell, calcium and mitochondrial imaging, viability assays, fluorescence activated cell sorting (FACS) analysis, Western blotting and immunohistochemistry. We confirmed that KGN cells produce H₂O₂

SUBMITTER: Hack CT 

PROVIDER: S-EPMC6912327 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature