Extrachromosomal Histone H2B Contributes to the Formation of the Abscission Site for Cell Division.

ABSTRACT: Histones are constitutive components of nucleosomes and key regulators of chromatin structure. We previously observed that an extrachromosomal histone H2B (ecH2B) localizes at the intercellular bridge (ICB) connecting the two daughter cells during cytokinesis independently of DNA and RNA. Here, we show that ecH2B binds and colocalizes with CHMP4B, a key component of the ESCRT-III machinery responsible for abscission, the final step of cell division. Abscission requires the formation of an abscission site at the ICB where the ESCRT-III complex organizes into narrowing cortical helices that drive the physical separation of sibling cells. ecH2B depletion does not prevent membrane cleavage rather results in abscission delay and accumulation of abnormally long and thin ICBs. In the absence of e

SUBMITTER: Monteonofrio L 

PROVIDER: S-EPMC6912571 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature