Distinguishing Anuran species by high-resolution melting analysis of the COI barcode (COI-HRM).

ABSTRACT: Taxonomic identification can be difficult when two or more species appear morphologically similar. DNA barcoding based on the sequence of the mitochondrial cytochrome c oxidase 1 gene (COI) is now widely used in identifying animal species. High-resolution melting analysis (HRM) provides an alternative method for detecting sequence variations among amplicons without having to perform DNA sequencing. The purpose of this study was to determine whether HRM of the COI barcode can be used to distinguish animal species. Using anurans as a model, we found distinct COI melting profiles among three congeners of both Lithobates spp. and Hyla spp. Sequence variations within species shifted the melting temperature of one or more melting domains slightly but do not affect the distinctness

SUBMITTER: Everman S 

PROVIDER: S-EPMC6912877 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature