Transcriptome assembly from long-read RNA-seq alignments with StringTie2.
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ABSTRACT:
SUBMITTER: Kovaka S
PROVIDER: S-EPMC6912988 | biostudies-literature | 2019 Dec
REPOSITORIES: biostudies-literature
Publications
Transcriptome assembly from long-read RNA-seq alignments with StringTie2.
Kovaka Sam S Zimin Aleksey V AV Pertea Geo M GM Razaghi Roham R Salzberg Steven L SL Pertea Mihaela M
Genome biology 20191216 1
RNA sequencing using the latest single-molecule sequencing instruments produces reads that are thousands of nucleotides long. The ability to assemble these long reads can greatly improve the sensitivity of long-read analyses. Here we present StringTie2, a reference-guided transcriptome assembler that works with both short and long reads. StringTie2 includes new methods to handle the high error rate of long reads and offers the ability to work with full-length super-reads assembled from short rea ...[more]
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