Increased Neuronal Differentiation Efficiency in High Cell Density-Derived Induced Pluripotent Stem Cells.

ABSTRACT: Human pluripotent stem cells (hPSCs), including induced pluripotent stem cells (iPSCs), provide access to hard-to-obtain cells for studies under physiological and disease conditions. For the study of neurodegenerative diseases, especially sporadic cases where the "disease condition" might be restricted towards the neuroectodermal lineage, obtaining the affected neurons is important to help unravel the underlying molecular mechanism leading to the diseases. Although differentiation of iPSCs to neural lineage allows acquisition of cell types of interest, the technology suffers from low efficiency leading to low yield of neurons. Here, we investigated the potential of adult neuroprogenitor cells (aNPCs) for iPSC derivation and possible confounders such as cell density of infected NPCs on thei

SUBMITTER: Srimasorn S 

PROVIDER: S-EPMC6913159 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature