Activity profiling of barley vacuolar processing enzymes provides new insights into the plant and cyst nematode interaction.

ABSTRACT: Vacuolar processing enzymes (VPEs) play an important role during regular growth and development and defence responses. Despite substantial attempts to understand the molecular basis of plant-cyst nematode interaction, the mechanism of VPEs functioning during this interaction remains unknown. The second-stage Heterodera filipjevi juvenile penetrates host roots and induces the formation of a permanent feeding site called a syncytium. To investigate whether infection with H. filipjevi alters plant host VPEs, the studies were performed in Hordeum vulgare roots and leaves on the day of inoculation and at 7, 14 and 21 days post-inoculation (dpi). Implementing molecular, biochemical and microscopic methods we identified reasons for modulation of barley VPE activity during interaction with H. fili

SUBMITTER: Labudda M 

PROVIDER: S-EPMC6913211 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature