Embryo aggregation regulates in vitro stress conditions to promote developmental competence in pigs.

ABSTRACT: Embryo aggregation is a useful method to produce blastocysts with high developmental competence to generate more offspring in various mammals, but the underlying mechanism(s) regarding the beneficial effects are largely unknown. In this study, we investigated the effects of embryo aggregation using 4-cell stage embryos in in vitro developmental competence and the relationship of stress conditions in porcine early embryogenesis. We conducted aggregation using the well of the well system and confirmed that aggregation using two or three embryos was useful for obtaining blastocysts. Aggregated embryos significantly improved developmental competence, including blastocyst formation rate, blastomere number, ICM/TE ratio, and cellular survival rate, compared to non-aggregated embryos. Inve

SUBMITTER: Jeong PS 

PROVIDER: S-EPMC6913270 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature