Effects of tranilast on the epithelial-to-mesenchymal transition in peritoneal mesothelial cells.

ABSTRACT:

Background

We investigated the effects of tranilast on epithelial-to-mesenchymal transition (EMT) in an animal model and on the EMT signaling pathway in human peritoneal mesothelial cells (HPMCs).

Methods

We performed in vitro studies (cytotoxicity, cell morphology, and western blot analyses) on HPMCs from human omenta, along with in vivo studies (peritoneal membrane function and morphometric and immunohistochemical analyses) on Sprague Dawley rats. Thirty-two rats were divided into three groups: control (C) group (peritoneal dialysis [PD] catheter but not infused with dialysate), PD group (4.25% glucose-containing dialysate), and PD + tranilast group (4.25% glucose-containing dialysate along with tranilast).

Results

In in vitro experiments, trans

SUBMITTER: Kang SH 

PROVIDER: S-EPMC6913598 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature