Increased performance of DNA metabarcoding of macroinvertebrates by taxonomic sorting.

ABSTRACT: DNA-based identification through the use of metabarcoding has been proposed as the next step in the monitoring of biological communities, such as those assessed under the Water Framework Directive (WFD). Advances have been made in the field of metabarcoding, but challenges remain when using complex samples. Uneven biomass distributions, preferential amplification and reference database deficiencies can all lead to discrepancies between morphological and DNA-based taxa lists. The effects of different taxonomic groups on these issues remain understudied. By metabarcoding WFD monitoring samples, we analyzed six different taxonomic groups of freshwater organisms, both separately and combined. Identifications based on metabarcoding data were compared directly to morphological assessments perfor

SUBMITTER: Beentjes KK 

PROVIDER: S-EPMC6913968 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature