Detection of Neutralizing Antibodies to Tembusu Virus: Implications for Infection and Immunity.

ABSTRACT: Neutralizing antibodies are the key mediators of protective immune response to flaviviruses after both infection and vaccination. Plaque reduction neutralization test (PRNT) is considered the "gold standard" for measurement of the immunity. To date, little is known regarding neutralizing antibody response to Tembusu virus (TMUV), a novel flavivirus emerging in ducks in 2010. Here, we developed a PRNT for detection of TMUV neutralizing antibodies. Following optimization and validation, the PRNT was applied to test serum samples from different flocks of ducks. Using sera prepared in experimental conditions, the levels of 50% end point titer (neutralizing dose, ND50) generated from positive sera (5,012-79,433) were significantly higher than those from mock-infected sera (10 to 126), indicatin

SUBMITTER: Lv J 

PROVIDER: S-EPMC6914806 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature