Identification and Verification of the Main Differentially Expressed Proteins in Gastric Cancer via iTRAQ Combined with Liquid Chromatography-Mass Spectrometry.

ABSTRACT:

Background

To find the potential intersections between the differentially expressed proteins and abnormally expressed genes in gastric cancer (GC) patients.

Methods

Gastric cancer tissue and adjacent normal mucosa tissue were used for iTRAQ analysis. Gene ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis, and protein-protein interaction (PPI) analysis were used to evaluate gene function. Western blotting and immunohistochemistry (IHC) were applied to verify the protein expression.

Results

A total of 2770 proteins were identified, of which 147 proteins were upregulated and 159 proteins were downregulated. GO analysis revealed that the differentially expressed genes were mainly enriched for the terms "cellular process," "binding," and "cell.

SUBMITTER: Gao Z 

PROVIDER: S-EPMC6914953 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature