Polymorphisms in Brucella Carbonic Anhydrase II Mediate CO₂ Dependence and Fitness in vivo.

ABSTRACT: Some Brucella isolates are known to require an increased concentration of CO₂ for growth, especially in the case of primary cultures obtained directly from infected animals. Moreover, the different Brucella species and biovars show a characteristic pattern of CO₂ requirement, and this trait has been included among the routine typing tests used for species and biovar differentiation. By comparing the differences in gene content among different CO₂-dependent and CO₂-independent Brucella strains, we have confirmed that carbonic anhydrase (CA) II is the enzyme responsible for this phenotype in all the Brucella strains tested. Brucella species contain two CAs of the β family, CA I and CA II; genetic polymorphisms exi

SUBMITTER: Garcia Lobo JM 

PROVIDER: S-EPMC6915039 | biostudies-literature | 2019

REPOSITORIES: biostudies-literature