Fibrillar Self-Assembly of a Chimeric Elastin-Resilin Inspired Engineered Polypeptide.

ABSTRACT: In the field of tissue engineering, recombinant protein-based biomaterials made up of block polypeptides with tunable properties arising from the functionalities of the individual domains are appealing candidates for the construction of medical devices. In this work, we focused our attention on the preparation and structural characterization of nanofibers from a chimeric-polypeptide-containing resilin and elastin domain, designed on purpose to enhance its cell-binding ability by introducing a specific fibronectin-derived Arg-Gly-Asp (RGD) sequence. The polypeptide ability to self-assemble was investigated. The molecular and supramolecular structure was characterized by Scanning Electronic Microscopy (SEM) and Atomic Force Microscopy (AFM), circular dichroism, state-of-the-art synchrotron r

SUBMITTER: Bracalello A 

PROVIDER: S-EPMC6915571 | biostudies-literature | 2019 Nov

REPOSITORIES: biostudies-literature