Colorectal cancers utilize glutamine as an anaplerotic substrate of the TCA cycle in vivo.

ABSTRACT: Cancer cells in culture rely on glutamine as an anaplerotic substrate to replenish tricarboxylic acid (TCA) cycle intermediates that have been consumed. but it is uncertain whether cancers in vivo depend on glutamine for anaplerosis. Here, following in vivo infusions of [¹³C₅]-glutamine in mice bearing subcutaneous colon cancer xenografts, we showed substantial amounts of infused [¹³C₅]-glutamine enters the TCA cycle in the tumors. Consistent with our prior observation that colorectal cancers (CRCs) with oncogenic mutations in the phosphatidylinositol-4,5-bisphosphate 3-kinase catalytic (PIK3CA) subunit are more dependent on glutamine than CRCs with wild type PIK3CA, labeling from glutamine to most TCA cycle intermediates was higher in PIK3CA-mut

SUBMITTER: Zhao Y 

PROVIDER: S-EPMC6915720 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature