Burn-related Collagen Conformational Changes in ex vivo Porcine Skin using Raman Spectroscopy.

ABSTRACT: This study utilizes Raman spectroscopy to analyze the burn-induced collagen conformational changes in ex vivo porcine skin tissue. Raman spectra of wavenumbers 500-2000 cm^-1 were measured for unburnt skin as well as four different burn conditions: (i) 200 °F for 10 s, (ii) 200 °F for the 30 s, (iii) 450 °F for 10 s and (iv) 450 °F for 30 s. The overall spectra reveal that protein and amino acids-related bands have manifested structural changes including the destruction of protein-related functional groups, and transformation from α-helical to disordered structures which are correlated with increasing burn severity. The deconvolution of the amide I region (1580-1720 cm^-1) and the analysis of the sub-bands reveal a change of the secondary structure of the collagen from

SUBMITTER: Ye H 

PROVIDER: S-EPMC6915721 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature