Scoring disease in an animal model of multiple sclerosis using a novel infrared-based automated activity-monitoring system.

ABSTRACT: Multiple sclerosis (MS) is a chronic demyelinating disorder of the central nervous system (CNS). Its corresponding animal model, experimental autoimmune encephalomyelitis (EAE), is widely used to understand disease pathogenesis and test novel therapeutic agents. However, existing methods to score EAE disease severity are subjective and often vary between individual researchers, making it difficult to translate findings across different studies. An enhanced automated method of disease scoring would eliminate subjectivity and reduce operator-dependent errors. Here, we used an Infra-Red Activity Monitoring System (IRAMS) to measure murine locomotor activity as a surrogate measure of disease severity and compared it to standard EAE scoring methods. In mice immunized with CNS-specific myelin an

SUBMITTER: Shahi SK 

PROVIDER: S-EPMC6915774 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature