N-methyl-D-aspartate (NMDA) receptor expression and function is required for early chondrogenesis.

ABSTRACT:

Background

In vitro chondrogenesis depends on the concerted action of numerous signalling pathways, many of which are sensitive to the changes of intracellular Ca²⁺ concentration. N-methyl-D-aspartate (NMDA) glutamate receptor is a cation channel with high permeability for Ca²⁺. Whilst there is now accumulating evidence for the expression and function of NMDA receptors in non-neural tissues including mature cartilage and bone, the contribution of glutamate signalling to the regulation of chondrogenesis is yet to be elucidated.

Methods

We studied the role of glutamatergic signalling during the course of in vitro chondrogenesis in high density chondrifying cell cultures using single cell fluorescent calcium imaging, patch clamp, transient gene silencing,

SUBMITTER: Matta C 

PROVIDER: S-EPMC6915923 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature