Associations between antimicrobial use and the faecal resistome on broiler farms from nine European countries.

ABSTRACT:

Objectives

To determine associations between farm- and flock-level antimicrobial usage (AMU), farm biosecurity status and the abundance of faecal antimicrobial resistance genes (ARGs) on broiler farms.

Methods

In the cross-sectional pan-European EFFORT study, conventional broiler farms were visited and faeces, AMU information and biosecurity records were collected. The resistomes of pooled faecal samples were determined by metagenomic analysis for 176 farms. A meta-analysis approach was used to relate total and class-specific ARGs (expressed as fragments per kb reference per million bacterial fragments, FPKM) to AMU (treatment incidence per DDD, TIDDDvet) per country and subsequently across all countries. In a similar way, the association between biosecurity status (Biocheck

SUBMITTER: Luiken REC 

PROVIDER: S-EPMC6916135 | biostudies-literature | 2019 Sep

REPOSITORIES: biostudies-literature