AKAP12 deficiency impairs VEGF-induced endothelial cell migration and sprouting.

ABSTRACT:

Aim

Protein kinase (PK) A anchoring protein (AKAP) 12 is a scaffolding protein that anchors PKA to compartmentalize cyclic AMP signalling. This study assessed the consequences of the downregulation or deletion of AKAP12 on endothelial cell migration and angiogenesis.

Methods

The consequences of siRNA-mediated downregulation AKAP12 were studied in primary cultures of human endothelial cells as well as in endothelial cells and retinas from wild-type versus AKAP12^-/- mice. Molecular interactions were investigated using a combination of immunoprecipitation and mass spectrometry.

Results

AKAP12 was expressed at low levels in confluent endothelial cells but its expression was increased in actively migrating cells, where it localized to lamellipodia. In the postna

SUBMITTER: Benz PM 

PROVIDER: S-EPMC6916389 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature