Trophectoderm regeneration to support full-term development in the inner cell mass isolated from bovine blastocyst.

ABSTRACT: Which comes first: tissue structure or cell differentiation? Although different cell types establish distinct structures delineating the inside and outside of an embryo, they progressively become specified by the blastocyst stage, when two types of cell lineages are formed: the inner cell mass (ICM) and the trophectoderm (TE). This inside-outside aspect can be experimentally converted by the isolation of the ICM from a blastocyst, leading to a posteriori externalization of the blastomeres composing the outermost layer of the ICM. Here, we investigated the totipotency of isolated mouse and bovine ICMs to determine whether they are competent for TE regeneration. Surprisingly, a calf was generated from the bovine isolated ICM with re-formed blastocoel (re-iICM), but no mouse re-iICMs d

SUBMITTER: Kohri N 

PROVIDER: S-EPMC6916479 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature