Proteome characterization of small extracellular vesicles from spared nerve injury model of neuropathic pain.

ABSTRACT: Exosomes are 30-150 nm extracellular vesicles mediating intercellular communication. Disease states can alter exosome composition affecting the message carried and thereby, its functional impact. The objective of this study was to identify proteins present in these vesicles in a mouse model of neuropathic pain induced by spared nerve injury (SNI). Small extracellular vesicles (sEVs) were purified from serum four weeks after SNI surgery and the protein composition was determined using tandem mass spectrometry and cytokine array. Proteomic analysis detected 274 gene products within sEVs. Of these, 24 were unique to SNI model, 100 to sham surgery control and five to naïve control samples. In addition to commonly expressed sEVs proteins, multiple members of serpin and complement family were de

SUBMITTER: Jean-Toussaint R 

PROVIDER: S-EPMC6916715 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature