Active and Repressed Chromatin Domains Exhibit Distinct Nucleosome Segregation during DNA Replication.

ABSTRACT: Chromatin domains and their associated structures must be faithfully inherited through cellular division to maintain cellular identity. However, accessing the localized strategies preserving chromatin domain inheritance, specifically the transfer of parental, pre-existing nucleosomes with their associated post-translational modifications (PTMs) during DNA replication, is challenging in living cells. We devised an inducible, proximity-dependent labeling system to irreversibly mark replication-dependent H3.1 and H3.2 histone-containing nucleosomes at desired loci in mouse embryonic stem cells so that their fate after DNA replication could be followed. Strikingly, repressed chromatin domains are preserved through local re-deposition of parental nucleosomes. In contrast, nucleosomes decorating

SUBMITTER: Escobar TM 

PROVIDER: S-EPMC6917041 | biostudies-literature | 2019 Oct

REPOSITORIES: biostudies-literature