Establishment of patient-derived orthotopic xenograft model of 1q+ posterior fossa group A ependymoma.

ABSTRACT:

Background

Treatment for pediatric posterior fossa group A (PFA) ependymoma with gain of chromosome 1q (1q+) has not improved over the past decade owing partially to lack of clinically relevant models. We described the first 2 1q+ PFA cell lines, which have significantly enhanced our understanding of PFA tumor biology and provided a tool to identify specific 1q+ PFA therapies. However, cell lines do not accurately replicate the tumor microenvironment. Our present goal is to establish patient-derived xenograft (PDX) mouse models.

Methods

Disaggregated tumors from 2 1q+ PFA patients were injected into the flanks of NSG mice. Flank tumors were then transplanted into the fourth ventricle or lateral ventricle of NSG mice. Characterization of intracranial tumors was performed usin

SUBMITTER: Pierce AM 

PROVIDER: S-EPMC6917412 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature