Recombinant polypeptide of Mycobacterium leprae as a potential tool for serological detection of leprosy.

ABSTRACT: Current prevention methods for the transmission of Mycobacterium leprae, the causative agent of leprosy, are inadequate as suggested by the rate of new leprosy cases reported. Simple large-scale detection methods for M. leprae infection are crucial for early detection of leprosy and disease control. The present study investigates the production and seroreactivity of a recombinant polypeptide composed of various M. leprae protein epitopes. The structural and physicochemical parameters of this construction were assessed using in silico tools. Parameters like subcellular localization, presence of signal peptide, primary, secondary, and tertiary structures, and 3D model were ascertained using several bioinformatics tools. The resultant purified recombinant polypeptide, designated rMLP15, is co

SUBMITTER: Barbosa MDS 

PROVIDER: S-EPMC6917672 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature