Multi-scale imaging and analysis identify pan-embryo cell dynamics of germlayer formation in zebrafish.

ABSTRACT: The coordination of cell movements across spatio-temporal scales ensures precise positioning of organs during vertebrate gastrulation. Mechanisms governing such morphogenetic movements have been studied only within a local region, a single germlayer or in whole embryos without cell identity. Scale-bridging imaging and automated analysis of cell dynamics are needed for a deeper understanding of tissue formation during gastrulation. Here, we report pan-embryo analyses of formation and dynamics of all three germlayers simultaneously within a developing zebrafish embryo. We show that a distinct distribution of cells in each germlayer is established during early gastrulation via cell movement characteristics that are predominantly determined by their position in the embryo. The differences in i

SUBMITTER: Shah G 

PROVIDER: S-EPMC6917746 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature