Characterization of a live-attenuated HCMV-based vaccine platform.

ABSTRACT: Vaccines based on cytomegalovirus (CMV) demonstrate protection in animal models of infectious disease and cancer. Vaccine efficacy is associated with the ability of CMV to elicit and indefinitely maintain high frequencies of circulating effector memory T cells (T_EM) providing continuous, life-long anti-pathogen immune activity. To allow for the clinical testing of human CMV (HCMV)-based vaccines we constructed and characterized as a vector backbone the recombinant molecular clone TR3 representing a wildtype genome. We demonstrate that TR3 can be stably propagated in vitro and that, despite species incompatibility, recombinant TR3 vectors elicit high frequencies of T_EM to inserted antigens in rhesus macaques (RM). Live-attenuated versions of TR3 were generated by delet

SUBMITTER: Caposio P 

PROVIDER: S-EPMC6917771 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature