PRIMEval: Optimization and screening of multiplex oligonucleotide assays.

ABSTRACT: The development of multiplex polymerase chain reaction and microarray assays is challenging due to primer dimer formation, unspecific hybridization events, the generation of unspecific by-products, primer depletion, and thus lower amplification efficiencies. We have developed a software workflow with three underlying algorithms that differ in their use case and specificity, allowing the complete in silico evaluation of such assays on user-derived data sets. We experimentally evaluated the method for the prediction of oligonucleotide hybridization events including resulting products and probes, self-dimers, cross-dimers and hairpins at different experimental conditions. The developed method allows explaining the observed artefacts through in silico WGS data and thermodynamic predictions. PR

SUBMITTER: Conzemius R 

PROVIDER: S-EPMC6917790 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature