GIRK1 triggers multiple cancer-related pathways in the benign mammary epithelial cell line MCF10A.

ABSTRACT: Excessive expression of subunit 1 of GIRK1 in ER⁺ breast tumors is associated with reduced survival times and increased lymph node metastasis in patients. To investigate possible tumor-initiating properties, benign MCF10A and malign MCF7 mammary epithelial cells were engineered to overexpress GIRK1 neoplasia associated vital parameters and resting potentials were measured and compared to controls. The presence of GIRK1 resulted in resting potentials negative to the controls. Upon GIRK1 overexpression, several cellular pathways were regulated towards pro-tumorigenic action as revealed by comparison of transcriptomes of MCF10A^GIRK1 with the control (MCF10A^eGFP). According to transcriptome analysis, cellular migration was promoted while wound healing and extra

SUBMITTER: Schratter G 

PROVIDER: S-EPMC6917815 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature