Gcn5-Mediated Histone Acetylation Governs Nucleosome Dynamics in Spermiogenesis.

ABSTRACT: During mammalian spermatogenesis, germ cell chromatin undergoes dramatic histone acetylation-mediated reorganization, whereby 90%-99% of histones are evicted. Given the potential role of retained histones in fertility and embryonic development, the genomic location of retained nucleosomes is of great interest. However, the ultimate position and mechanisms underlying nucleosome eviction or retention are poorly understood, including several studies utilizing micrococcal-nuclease sequencing (MNase-seq) methodologies reporting remarkably dissimilar locations. We utilized assay for transposase accessible chromatin sequencing (ATAC-seq) in mouse sperm and found nucleosome enrichment at promoters but also retention at inter- and intragenic regions and repetitive elements. We further generated ger

SUBMITTER: Luense LJ 

PROVIDER: S-EPMC6917834 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature