Injection molded open microfluidic well plate inserts for user-friendly coculture and microscopy.

ABSTRACT: Open microfluidic cell culture systems are powerful tools for interrogating biological mechanisms. We have previously presented a microscale cell culture system, based on spontaneous capillary flow of biocompatible hydrogels, that is integrated into a standard cell culture well plate, with flexible cell compartment geometries and easy pipet access. Here, we present two new injection molded open microfluidic devices that also easily insert into standard cell culture well plates and standard culture workflows, allowing seamless adoption by biomedical researchers. These platforms allow culture and study of soluble factor communication among multiple cell types, and the microscale dimensions are well-suited for rare primary cells. Unique advances include optimized evaporation control within th

SUBMITTER: Day JH 

PROVIDER: S-EPMC6917835 | biostudies-literature | 2020 Jan

REPOSITORIES: biostudies-literature