Microglia Adopt Longitudinal Transcriptional Changes After Traumatic Brain Injury.

ABSTRACT:

Background

Traumatic brain injury (TBI) is an under-recognized public health threat. Even mild brain injuries can lead to long-term neurologic impairment. Microglia play a fundamental role in the development and progression of this ensuing neurologic impairment. Despite this, a microglia-specific injury signature has yet to be identified. We hypothesized that TBI would lead to long-term changes in the transcriptional profile of microglial pathways associated with the development of subsequent neurologic impairment.

Materials and methods

Male C57BL/6 mice underwent TBI via a controlled cortical impact and were followed longitudinally. FACSorted microglia from TBI mice were subjected to Quantiseq 3'-biased RNA sequencing at 7, 30, and 90 d after TBI. K-means clustering on 396

SUBMITTER: Makinde HM 

PROVIDER: S-EPMC6917875 | biostudies-literature | 2020 Feb

REPOSITORIES: biostudies-literature