Subcellular NAMPT-mediated NAD⁺ salvage pathways and their roles in bioenergetics and neuronal protection after ischemic injury.

ABSTRACT: NAD⁺ is a cofactor required for glycolysis, tricarboxylic acid cycle, and complex I enzymatic reaction. In mammalian cells, NAD⁺ is predominantly synthesized through the salvage pathway, where nicotinamide phosphoribosyltransferase (NAMPT) is the rate-limiting enzyme. Previously, we demonstrated that NAMPT exerts a neuroprotective effect in ischemia through the suppression of mitochondrial dysfunction. Mammalian cells maintain distinct NAD⁺ pools in the cytosol, mitochondria, and nuclei. However, it is unknown whether mitochondria have an intact machinery for NAD⁺ salvage, and if so, whether it plays a dominant role in bioenergetics, mitochondrial function, and neuronal protection after ischemia. Here, using mouse primary cortical neuron and cort

SUBMITTER: Wang X 

PROVIDER: S-EPMC6917901 | biostudies-literature | 2019 Dec

REPOSITORIES: biostudies-literature